Datasets from a vapor diffusion mineral precipitation protocol for Dictyostelium stalks
نویسندگان
چکیده
Datasets from a slow carbonate vapor diffusion and mineral precipitation protocol for Dictyostelium ECM and cellulose stalks show examples for composite materials obtained by an in vitro approach, which differs substantially from the in vivo approach reported in The Journal of Structural Biology, doi: 10.1016/j.jsb.2016.03.015 [1]. Methods for obtaining the datasets include bright field transmitted light microscopy, fluorescence microscopy, LC-PolScope birefringence microscopy, variable pressure scanning electron microscopy (VP-SEM/ESEM), and Raman imaging spectroscopy.
منابع مشابه
Loss of the β-catenin homologue aardvark causes ectopic stalk formation in Dictyostelium
Aardvark (Aar) is a Dictyostelium beta-catenin homologue with both cytoskeletal and signal transduction roles during development. Here, we show that loss of aar causes a novel phenotype where multiple stalks appear during late development. Ectopic stalks are preceded by misexpression of the stalk marker ST-lacZ in the surrounding tissue. This process does not involve the kinase GSK-3. Mixing ex...
متن کاملLattice Boltzmann simulation of snow crystal growth in clouds
[1] The Lattice Boltzmann (LB) method can be used to simulate aspects of chemical reactions such as mineral precipitation from a fluid phase or condensation from a vapor phase. The LB method has the advantage of allowing the shape of the condensing phase to evolve depending on local conditions rather than being specified, so that the controls on condensed phase grain shape can be studied simult...
متن کاملProtein crystallization for X-ray crystallography.
Using the three-dimensional structure of biological macromolecules to infer how they function is one of the most important fields of modern biology. The availability of atomic resolution structures provides a deep and unique understanding of protein function, and helps to unravel the inner workings of the living cell. To date, 86% of the Protein Data Bank (rcsb-PDB) entries are macromolecular s...
متن کاملDictyostelium AMPKa regulates aggregate size and cell-type patterning
Starved Dictyostelium cells aggregate into groups of nearly 10 cells. AMPK is a highly conserved serine/threonine protein kinase consisting of a catalytic and two regulatory subunits. As multi-cellular development in Dictyostelium is initiated upon starvation, we explored the role of the energy sensor, AMPK, which shows significant similarity to human AMPK and is expressed throughout developmen...
متن کاملDictyostelium AMPKα regulates aggregate size and cell-type patterning
Starved Dictyostelium cells aggregate into groups of nearly 105 cells. AMPK is a highly conserved serine/threonine protein kinase consisting of a catalytic and two regulatory subunits. As multi-cellular development in Dictyostelium is initiated upon starvation, we explored the role of the energy sensor, AMPK, which shows significant similarity to human AMPK and is expressed throughout developme...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره 7 شماره
صفحات -
تاریخ انتشار 2016